The RNA Detection Reagent is used for the qualitative detection of the S and N genes in the throat swab and sputum sample. This kit uses One-Step Real-Time PCR and TaqMan-MGB fluorescent probe technology. Primers and probes are designed in the highly conserved regions of the S and N gene sequences of it, and human RNase P gene as internal control. This kit has been validated on samples extracted from sputum, nasopharyngeal and throat swabs. Three fluorescent reporters are incorporated into this test: FAM measures its S gene; VIC measures its N gene; CY5 measures human RNase P gene as internal control. The kit reagents should be stored at -20±10℃ in the dark in a freezer. When properly stored, the reagents are stable for 12 months when sealed and 2 months after initial use. Avoid and reduce repeated freezing and thawing. The reagents should be discarded after 5 times of freezing and thawing. If reagents will be used intermittently, it is suggested to keep the reagents frozen in aliquots.The qualitative detection of the S and N genes in the throat swab and sputum sample.

Name	(50 rxns)	(100 rxns)	Component
SolutionⅠ	150μl	300μl	Primer and Probe
SolutionⅡ	600μl	1200μl	Mix
Positive	100μl	200μl	Plasmids containing target gene fragments
Negative	100μl	200μl	RNase Free water
* dNTP, Buffer MgSO4, Taq DNA Polymerase, Rox, Reverse Transcriptase